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Extra resources for Current Protocols in Cytometry
Resolution: degree to which a ﬂow cytometry measurement parameter can distinguish two populations in a mixture of particles that differ in mean signal intensity. Fluorescence sensitivity (see above) can be considered a special case of ﬂuorescence resolution for which the signals are very dim. Note that the resolution will appear different when data are acquired and/or displayed on a logarithmic rather than linear intensity scale. , 256 or 1024), a logarithmic display of the data may not have sufﬁcient resolution to display populations that can actually be resolved by the instrument.
Antibody binding capacity (ABC) Standardization, Calibration, and Control in Flow Cytometry A further step in immunoﬂuorescence standardization and calibration is to express measurement results in terms of antibody binding capacity. Three approaches have been used to estimate ABC. Each approach has different critical technical requirements and potential sources of error. Although not, strictly speaking, a source of error, it must be kept in mind that different antibody clones with the same cluster designation (CD) can have different binding capacity.
6 Current Protocols in Cytometry withstand the mechanical rigors of the sorting process and by the ability of the fluidic plumbing to withstand the higher pressures required. For historical reasons, commercial sorters have been limited to operating pressures of ~1 atm (15 psi) and nozzle sizes of ~70 to 100 µm, which meant that drop frequencies have been limited to ~25,000–35,000/sec. More recently, sorters have become available that operate at significantly higher pressure and correspondingly higher jet velocity and drop frequency.
Current Protocols in Cytometry by Robinson