By Thomas G., II Pretlow, Theresa P. Pretlow
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Additional info for Cell Separation. Methods and Selected Applications, Volume 2
Muscoplat, C. C , Shoster, J. , Osborne, C. , and Johnson, D. W. (1977). Density gradient separation of lymphocytes, eosinophils and microfilariae from blood of dogs infected with Dirofilaria immitis. Am. J. Vet. 38, 2095-2096. , Nogueira-Machado, J. , and Gazzinelli, G. (1980). Schistosoma mansoni: Comparison of the killing effect of granulocytes and complement with or without antibody on fresh and cultured schistosomula in vitro. Am. J. Trop. Med. Hyg. 29, 1263-1267. , and Venge, P. (1979). The role of the eosinophil in granulocyte in the inflammatory reaction.
Immunol. Methods 27, 373-382. James, S. , Lazdins, J. , and Meltzer, M. S. (1982). Macrophages as effector cells of protective immunity in murine schistosomasis. II. Killing of newly transformed schistosomula in vitro by macrophages activated as a consequence of Schistosoma mansoni infection. J. Immunol 128, 1535-1540. Johnson, G. , Nicholas, W. , McKenzie, I. F. C , and Mitchell, G. F. (1979). Peritoneal cell population of mice infected with Mesocestoides corti as a source of eosinophils. Int.
2x 107/cells/hour (or 2x 104 cells/second) can be analyzed. IV. Summary and Conclusions The current state-of-the-art for purification of eosinophils has been reviewed. Numerous approaches have been taken exploiting differences in cell density, membrane properties, phagocytic abilities, and autofluorescence. Of these the most widely used is the density difference between eosinophils and other cells. Presently, the mostly widely used method is sedimentation through Metrizamide although sedimentation in polyvinylpyrrolidone-coated silica particles (Percoll) also provides highly purified eosinophils in good yield.
Cell Separation. Methods and Selected Applications, Volume 2 by Thomas G., II Pretlow, Theresa P. Pretlow